bp5
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Immunocytochemistry analysis was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 stained by 53BP1 Antibody (Product # MA5-36262) diluted at 1:1,000. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
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Immunohistochemistry (Paraffin) analysis performed in paraffin-embedded human breast carcinoma. 53BP1 stained by 53BP1 Antibody (Product # MA5-36262) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Western Blot using 53BP1 Monoclonal Antibody (HL275) (Product # MA5-36262). HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 Polyclonal Antibody [HL36] 53BP1 Monoclonal Antibody (HL275) (Product # MA5-36262) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 Antibody (Product # MA5-36262) diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Western Blot using 53BP1 Monoclonal Antibody (HL275) (Product # MA5-36262). Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 Polyclonal Antibody [HL36] 53BP1 Monoclonal Antibody (HL275) (Product # MA5-36262) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 Antibody (Product # MA5-36262) diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 Antibody (Product # MA5-36262) diluted at 1:1,000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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at 1:1,000. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
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